The MicroCal PEAQ-ITC delivers direct determination of all binding parameters and thermodynamic parameters in a single experiment. With minimal sample preparation and system optimization, data is generated quickly and easily. The wide affinity range enables analysis of weak to high affinity binders, with excellent reproducibility.
It is easy to handle, help videos in the software guide the operator and the semi-automated measurement set-up and cleaning procedures minimize outside intervention.

MicroCal ITC is an essential tool for any research laboratory studying biomolecular interactions where high sensitivity and fast results are paramount.

To meet the needs of today’s life science laboratories a wide range of applications can be investigated with MicroCal PEAQ-ITC, including characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics.

The system is upgradable to the fully automated MicroCal PEAQ-ITC Automated

Features and benefits of MicroCal PEAQ-ITC

• User-friendly guided workflows with embedded help videos give any level of user the ability to generate high quality data
• High signal to noise gives more confidence in accessing data quality and relevance of generated affinity and thermodynamic parameters
• Automated washing with detergent of the sample cell and titration syringe assists in producing high quality reproducible data
• All binding parameters (affinity, stoichiometry, enthalpy and entropy) in a single experiment
• Quick to first results with no assay development, no labelling, no immobilization and no limitations due to molecular weight or optical properties
• Sensitivity to investigate any biomolecular interaction using as little as 10µg of protein
• Directly measures millimolar to nanomolar affinities (K_DS 10^-2 to 10^-9 M)
• Measures nanomolar to picomolar disassociation constants
  using competitive binding techniques (10^-9 to 10^-12M)
• Nonreactive Hastelloy for chemical resistance and compatibility with biological samples
• Compatible with non-aqueous solvents
• Upgradable to fully automated MicroCal PEAQ-ITC Automated

PEAQ-ITC Software

The PEAQ-ITC software offers experiment design simulation, batch evaluation of large data sets, automated assessment of data quality and a streamlined user interface that guides the user to final figures and presentation quality graphs quickly and easily.

• Open multiple experiments in a single session
• Supports many automated fitting models
  • Dissociation
  • Enzyme Kinetics – Multiple Injections
  • Enzyme Kinetics – Single Injection
  • One Set of Sites
  • Sequential Binding Sites
  • One Set of Sites – SIM
  • Two Sets of Sites
  • Competitive
• Automated assessment of data quality
  • Good quality data – Binding
  • Good quality data – No binding
  • Poor quality data – Check data