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MicroCal PEAQ-ITC Automated

The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics.

Infos / Datasheet

The MicroCal PEAQ-ITC Automated combines the high sensitivity of the MicroCal PEAQ-ITC – the calorimeter core of the system – with walkaway automation, to meet the productivity needs of busy research and drug discovery laboratories.

The system directly measures heat released or absorbed during biochemical binding events, from which it calculates binding affinity (KD), stoichiometry (n), enthalpy (ΔH), and entropy (ΔS). Other than preparing samples and reagents to appropriate concentrations, there is minimal assay development and time to first result is fast. Easy-to use software includes optimized automation scripts for robust operation with a variety of sample types and conditions.

Features and benefits

  • User-friendly design tools assist in selecting experimental run parameters
  • All binding parameters (affinity, stoichiometry, enthalpy and entropy) in a single experiment
  • Quick to first result with minimal assay development, no labelling, no immobilization and no limitations due to molecular weight or optical properties
  • High signal to noise gives more confidence in accessing data quality and relevance of generated affinity and thermodynamic parameters
  • Outstanding data quality for sub-millimolar to picomolar disassociation constants (10-2 to 10-12 M)
  • Coin shaped cell optimizes sample mixing
  • Nonreactive Hastelloy for chemical resistance and compatibility with biological samples
  • Sensitivity to investigate any biomolecular interaction using as little as 10µg of protein
  • Samples presented in temperature controlled 96-well plate
  • Fully automated system with walk-away operation for enhanced productivity

PEAQ ITC Software

MicroCal PEAQ-ITC software offers experiment design simulation, batch evaluation of large data sets, automated assessment of data quality and a streamlined user interface that guides the user to final figures and presentation quality graphs quickly and easily.

  • Open multiple experiments in a single session
  • Supports many automated fitting models
    • Dissociation
    • Enzyme Kinetics – Multiple Injections
    • Enzyme Kinetics – Single Injection
    • One Set of Sites
    • Sequential Binding Sites
    • One Set of Sites – SIM
    • Two Sets of Sites
    • Competitive
  • Automated assessment of data quality
    • Good quality data – Binding
    • Good quality data – No binding
    • Poor quality data – Check data