Publication-quality binding kinetics & affinity data on your benchtop.

OpenSPR is the world’s only benchtop surface plasmon resonance (SPR) instrument. It provides high quality, label-free interaction analysis for a fraction of the cost of existing solutions. Our unique nano-structured sensor surface uses localized SPR (LSPR) to deliver repeatable, highly sensitive kinetic data.

• OpenSPR Datasheet

What is Surface Plasmon Resonance (SPR)?

SPR is an optical effect that can be utilized to measure the binding kinetics and affinity of molecular interactions in real-time without the use of labels.
SPR is one of the few techniques that allows determination of binding kinetics k_on and und k_off as well as binding affinity K_D.

How is LSPR different from SPR?

Localized surface plasmon resonance is generated by gold nanoparticles as compared to a continuous gold film as used in traditional SPR.
LSPR produces a strong resonance absorbance peak in the visible range of light, with it’s position being highly
sensitive to the local refractive index surrounding the particle. Therefore, OpenSPR measures small changes in
the wavelength of the absorbance position, rather than a reflected angle as in traditional SPR.

Advantages of using LSPR instead of SPR

• The optical hardware for LSPR is less complex, so the instrument is smaller and more affordable
• Since the angle is not important, the instrument is more robust against vibration and mechanical noise
• LSPR is not as sensitive to bulk sample effects, which causes errors in experimental data, because it has a much shorter electromagnetic field decay length
• No strict temperature control is needed
• The sensor chips can be manufactured at a much more affordable price
• Instruments are easier to use and maintain

OpenSPR Systems are primarily used for affinity characterization respectively to determine Binding Kinetics of Proteins, Lipids, Carbohydrates, Antibodies, Nucleic Acids, Small Molecules, Cells, Viruses and Nanoparticles with the aim to use this data for Competition Assays, Target Identification, Epitope Mapping and for Screening in general.